PMC

The figure shows the position of the reaction product bound at the active site of the enzyme. The proximity of the C5 group to Ala85 demonstrates how the removal of the threonine side chain has provided extra space to accommodate the allyl group.

The product space for threshold ; each edge links two products that have a proximity larger than . The red nodes represent the core products which we define later.

Left, hot-spot pressure and energy. The product P²E_hs is representative of proximity to ignition; contours of this metric relative to N210207 are shown by the dashed grey curves. Right, yield amplification (Y_amp) versus ITFX_nα. These are the highest performing ICF experiments so far and the closest to ignition. The inset shows these experiments in detail with both inferred (solid) and simulated (open) Y_amp. Error bars are 1 s.d. and are shown only for the I-Raum and Hybrid-E points, plus shot N180128. Historical data are from refs. ^,,,–,–.

Electron tomography of a 250-nm section of G. lamblia labeled for G6P detection. The image on the left is from a single digital slice (2.7 nm) showing reaction product on the nuclear envelope, the TVN, and some PVs (arrow). An arrowhead (lower center below the nucleus) points to a proximity point of the TVN system and the nuclear envelope. On the right is a false-color 3D model of the same cell showing the widespread distribution of TVN tubules (yellow) and cisternae (green) that fill most of the space between the nuclei and the PVs that are positive for G6P (blue). When seen in cross section, the TVN appears vesicular (V), while in longitudinal view it appears tubular (T). Elements of the ventral disk are depicted in light blue. N, nucleus; f, flagellum. The insets show the correlated appearance of the TVN (ER), PVs (PV), and perinuclear (PN) cisternae of the TVN in confocal microscopic slices that are also shown in Fig. , , and . Scale bar, 500 nm.